Cytotoxic T-lymphocyte elicited vaccine against SARS-CoV-2 employing immunoinformatics framework

Abstract Development of effective counteragents against the novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) strains, requires clear insights and information for understanding immune responses associated with it. This global pandemic has pushed healthcare system restricted movement people succumbing available therapeutics utterly warrants development a potential vaccine to contest deadly situation. In present study, highly efficacious, immunodominant cytotoxic T-lymphocyte (CTL) epitopes were predicted advanced immunoinformatics assays using spike glycoprotein SARS-CoV2, generating robust specific response convincing immunological parameters (Antigenicity, TAP affinity, MHC binder) engendering an efficient viral vaccine. The molecular docking studies show strong binding CTL construct MHC-1 host membrane TLR2 receptors. dynamics simulation in explicit confirmed stable epitope TLR2. Steep magnitude RMSD variation compelling residual fluctuations existed terminal residues various loops β linker segments TLR2-epitope (residues 105-156 239-254) about 0.4 nm. reduced R g value (3.3 nm) stagnant SASA analysis (275 nm/S /N after 8 ns 5 ns) protein surface its orientation exposed buried regions suggests more compactness due interaction epitope. candidate establishes high capability elicit critical regulators, like T-cells memory cells as proven silico immunization can be further corroborated through vitro vivo assays.